Geomicrobiology of Antarctic Subglacial Environments - Subglacial Lake Whillans

http://ipt.biodiversity.aq/resource.do?r=gbase
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organizationName Montana State University
bounding Coordinates {"south":"-85.02","north":"-83.79","west":"-155.33","east":"-131.77"}
abstract Liquid water has been known to occur beneath the Antarctic ice sheet for more than 40 years,but only recently have these subglacial aqueous environments been recognized as microbial ecosystems that may influence biogeochemical transformations on a global scale. Here we present the first geomicrobiological description of water and surficial sediments obtained from direct sampling of a subglacial Antarctic lake. Subglacial Lake Whillans (SLW)lies beneath approximately 800m of ice on the lower portion of the Whillans Ice Stream (WIS) in West Antarctica and is part of an extensive and evolving subglacial drainage network. The water column of SLW contained metabolically active microorganisms and was derived primarily from glacial ice melt with solute sources from lithogenic weathering and a minor seawater component. Heterotrophic and autotrophic production data together with small subunit ribosomal RNA gene sequencing and biogeochemical data indicate that SLW is a chemosynthetically driven ecosystem inhabited by a diverse assemblage of bacteria and archaea. Our results confirm that aquatic environments beneath the Antarctic ice sheet support viable microbial ecosystems, corroborating previous reports suggesting that they contain globally relevant pools of carbon and microbes that can mobilize elements from the lithosphere and influence Southern Ocean geochemical and biological systems.

MiMarks Samples

SLW_MC-2B_30Jan2013
submitted_to_insdc investigation_type experimental_factor
lat_lon collection_date env_biome
env_material env_package subspecf_gen_lin
source_mat_id biotic_relationship
rel_to_oxygen isol_growth_condt samp_collect_device
nucl_acid_ext nucl_acid_amp lib_size
lib_const_meth lib_vector lib_screen
target_subfragment seq_meth pcr_primers
adapters mid seq_quality_check
assembly sop
depth depth_unit
bacteria_carb_prod_leu bacteria_carb_prod_leu_unit bacteria_carb_prod_thy
carb_nitro_ratio
part_org_carb part_org_carb_unit part_org_nitro
SLW_MC-3B_30Jan2013
submitted_to_insdc investigation_type experimental_factor
lat_lon collection_date env_biome
env_material env_package
depth depth_unit
bromide bromide_unit calcium
chloride chloride_unit
magnesium magnesium_unit
nitrate nitrate_unit
ph
phosphate phosphate_unit
potassium potassium_unit
sodium sodium_unit sulfate
delta_18O delta_18O_unit bicarbonate

Sequence sets

SLW_CastN_27Jan13
collection_date study_type target_gene
region_targeted forward_primer reverse_primer
reverse_primer_sequence primer_5 primer_3
sequencing_technology seq_yr run_type
sequence_data_repository url_data_repository
sra_accession_number sra_project_number sra_run_number
number_of_spots_in_sra number_of_bases_predicted dataset_id
updated_at
SLW_Cast1_28Jan13
collection_date study_type target_gene
region_targeted forward_primer reverse_primer
reverse_primer_sequence primer_5 primer_3
sequencing_technology seq_yr run_type
sequence_data_repository url_data_repository
sra_accession_number sra_project_number sra_run_number
number_of_spots_in_sra number_of_bases_predicted dataset_id
updated_at
SLW_Cast2_30Jan13
collection_date study_type target_gene
region_targeted forward_primer reverse_primer
reverse_primer_sequence primer_5 primer_3
sequencing_technology seq_yr run_type
sequence_data_repository url_data_repository
sra_accession_number sra_project_number sra_run_number
number_of_spots_in_sra number_of_bases_predicted dataset_id
updated_at
SLW_Cast3_30Jan13
collection_date study_type target_gene
region_targeted forward_primer reverse_primer
reverse_primer_sequence primer_5 primer_3
sequencing_technology seq_yr run_type
sequence_data_repository url_data_repository
sra_accession_number sra_project_number sra_run_number
number_of_spots_in_sra number_of_bases_predicted dataset_id
updated_at
SLW_MC-2B_30Jan2013
collection_date study_type target_gene
region_targeted forward_primer reverse_primer
reverse_primer_sequence primer_5 primer_3
sequencing_technology seq_yr run_type
sequence_data_repository url_data_repository
sra_accession_number sra_project_number sra_run_number
number_of_spots_in_sra number_of_bases_predicted dataset_id
updated_at
SLW_MC-3B_30Jan2013
collection_date
dataset_id created_at updated_at
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